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Protein Discovery Introduces PPS Silent™ Surfactant

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Protein Discovery, Inc. has announced the commercial introduction of PPS Silent™ Surfactant, an acid-cleavable detergent for cell membrane disruption and protein solubilization.

PPS Silent Surfactant will be marketed to proteomics researchers analyzing low-abundance cellular proteins using mass spectrometry.

The reagent is designed to offer the same detergent properties as conventional surfactants, but when it is no longer needed, it degrades into removed compounds with no detergent activity.

Protein Discovery claims that, unlike conventional detergents, which retain foaming and other detergent properties that interfere with the final stages of mass spectrometry sample preparation, PPS Silent Surfactant does not diminish spectral quality or protein detection sensitivity.

The announcement marks Protein Discovery's entry into the market for mass spectrometry sample preparation reagents.

"PPS Silent Surfactant definitely helps with the recovery and digestion of low abundance proteins for shotgun proteomics," said beta tester Michael MacCoss PhD, assistant professor at University of Washington's Department of Genome Sciences.

"It clearly outperforms everything else we've used to generate peptides from complex protein mixtures."

"The cleavage products of Protein Discovery's detergent are still soluble after acid hydrolysis, so you don't have to try to spin out leftover insoluble residue before analyzing the peptides with nanoLC-MS/MS," he added.

"PPS Silent Surfactant is an aggressive detergent which can be used both for membrane disruption and protein solubilization, so researchers don't lose time and sample yields by having to change out their detergent solutions halfway through the experiment," said Jeremy Norris PhD, director of mass spectrometry at Protein Discovery.

"After cleavage, there's no residual detergent activity to get in the way of mass spec analysis."