Combatting the COVID-19 pandemic requires greater insights into the molecular epidemiology of SARS-CoV-2 to trace existing outbreaks, study viral evolution and advance vaccine and drug development research. To support these efforts, we’ve developed the new QIAseq SARS-CoV-2 Primer Panel – an NGS-based solution for targeted enrichment of the complete viral genome. Multiplex up to 384 samples using a faster workflow, and rapidly analyze your data with integrated analysis pipelines. Based on optimized publicly-available ARTIC V3 designs, the QIAseq SARS-CoV-2 Primer Panel minimizes primer dropouts and primer-dimer effects. Combine with the QIAseq FX DNA Library UDI Kit to convert amplicons into NGS-ready libraries compatible with Illumina platforms.
Achieve high library complexity and maximize coverage with our optimized, single-day workflows, which are faster and have lower-input requirements compared to hybrid capture solutions. Benefit from a fast 3–3.5 hour enrichment workflow with limited hands-on time.
Key performance attributes:
- High-quality, single-box solution Targeting SARS-CoV-2 requires both reverse transcription and whole genome enrichment of the viral RNA. The QIAseq SARS-CoV-2 Primer Panel combines both of these steps to generate amplicons for downstream library creation. When paired with a QIAseq FX DNA Library UDI Kit, you can construct sequencing-ready libraries compatible with Illumina platforms.
- Community primer design QIAseq SARS-CoV-2 primer pools utilize designs from the ARTIC V3 primers. The primers have also gone through an in silico check to reduce chances for dimerization during sample enrichment.
- Multiplexing Combine with a QIAseq FX DNA Library UDI Kit to enable multiplexing on high-throughput Illumina instruments, such as the NovaSeq (up to 384 samples per flow cell).
- Data analysis When paired with QIAGEN CLC Genomics Workbench, the panel can deliver data that can be quickly analyzed. Sequence data can be used to identify variants across different samples, and compared to multiple genomes – from consensus reference genomes to one of many genomes that have been uploaded from around the world. For a limited time only, you can receive 25% off any 1-year subscription to QIAGEN CLC Genomics software with every purchase of the QIAseq SARS-CoV-2 Primer Panel Kit (96).