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Vutara VXL Super-Resolution Microscope

Vutara VXL Super-Resolution Microscope

More than the leading single-molecule localization microscope (SMLM), Bruker’s Vutara VXL is a comprehensive workstation for nanoscale biological imaging. Both core facilities and individual investigators utilize this affordable and easy-to-use technology to perform compelling research in a variety of disciplines such as biophysics, cell biology, and neuroscience. The Vutara VXL is a streamlined and compact system equipped with industry leading SMLM technology. Researchers utilize this novel system to perform research on DNA, RNA, and proteins, from macromolecular complexes and super-structures to chromatin structures and chromosomal substructures to studying functional relationships in genomes and in various subcellular organelles. The Vutara VXL also supports advanced spatial biology research in extracellular matrix structures, extracellular vesicles (EV), virology, neuroscience, and live-cell imaging.

Bruker’s SMLM technology collects data from individual molecules by turning fluorophores on and off one at a time, causing the center position of each molecule to be recorded separately. By stochastically exciting individual molecules at separate time points during image acquisition, users can gather specific localization information. Therefore, overcoming many of the barriers researchers face when using traditional microscopy techniques. This SMLM approach delivers the highest resolution, down to 20nm laterally. Vutara’s proprietary biplane technology also offers researchers the deepest 3D imaging with depths of up to 50 μm while top-hat illumination enables uniform, quantitative data collection. Additionally, robust flexible software creates seamless multimodal imaging for studies across length scales. When combined with Bruker’s unique microscope fluidics unit, PlexFlo, users get access to easy yet unlimited multiplexed imaging for spatial genomics, transcriptomics, and proteomics.

The Vutara VXL is capable of imaging far from the surface of the coverslip to accommodate a wide range of sample types. Proprietary bi-plane technology, with widefield illumination up to 100 μm, and SRX software, lets users obtain single-molecule data on more sample types than any other commercial SML microscope. Samples and structures include synapses, nuclei, viruses, cell membranes, cell cultures, cell colonies, tissue sections, and even whole organisms. Vutara’s Quantitative Localization Microscopy suite allows you to turn localizations and voxels into meaningful results by guiding users through the setup, calibration, imaging, processing, and analysis of the super-resolution single-molecule localization experiments that include spatial distributions, cluster analyses, colocalizations, resolution analyses, and live-cell analyses. By combining real-time localization processing with powerful 3D visualization and analysis tools, the SRX software lets researchers quickly create publication-quality videos, images, and measurements.

The Vutara VXL also enables deep investigation for diverse applications. SMLM can be combined with other techniques, such as DNA-PAINT, OligoSTORM, and smFISH, to enable previously unobtainable data like point accumulation in nanoscale topography as well as imaging and quantifying RNA. Among super-resolution methods, SMLM offers the best improvement in resolution along with quantitative and statistical analysis capabilities, making the Vutara VXL a powerful workstation for enabling breakthrough discoveries in the life sciences.

Product Specifications
Imaging Modalities SMLM with high z-resolution for STORM, PALM, PAINT, and related super-resolution applications
Flat illumination Flat excitation profile guaranteed by top hat illumination from a square fiber
Multi-color acquisition Up to 5 colors sequential
Multi-plane imaging Simultaneous imaging of two focal planes allows 1 µm depth discrimination
Objective 60x magnification; 1.3 numerical aperture (NA); Silicon oil immersion; 0.3 mm working distance; #1.5H cover glass (0.170 ±0.005 mm)
Field of View (FOV) 200 µm x 200 µm for multiplexed localization microscopy and widefield imaging; 50 µm x 50 µm for SMLM with switching (STORM, PALM, PAINT) and 3D localization (biplane detection); Larger FOV with tile scanning
SMLM resolution 20 nm laterally (xy); 50 nm axially (z) with biplane
Imaging depth > 30 µm (typical, dependent on sample)
xy-stage Easy access to sample; 100 x 50 nm travel range; Optical encoder with 1 nm resolution
z-focus Course focus to localize sample; Fine focus for fast z-stack acquisition