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Anticancer Therapies – Multimedia

Cytosolic Delivery of Anticancer Drug Using Endosomolytic Peptide Bearing Nanoparticles Against Solid Tumor content piece image
Poster

Cytosolic Delivery of Anticancer Drug Using Endosomolytic Peptide Bearing Nanoparticles Against Solid Tumor

The objective of the present project is to develop transferrin coupled biodegradable nanoparticles (NPs) with high encapsulation efficiency (Doxorubicin). GALA (Glutamic acid-alanine-leucine alanine) a pH sensitive fusogenic endosomolytic peptide is added to the formulation to enhance the endosomal lysis that will further potentiate the delivery of anticancer drug inside tumor cell as well as prevent loss of drug via endosomes.
Sub-classification of Colorectal Cancer Using Surface Antigen Antibody Microarray and Fluorescence Multiplexing content piece image
Poster

Sub-classification of Colorectal Cancer Using Surface Antigen Antibody Microarray and Fluorescence Multiplexing

Colorectal cancer (CRC) is the second most frequent cause of cancer deaths in Australia. Even after resection up to 50% of patients relapse. In an attempt to prevent recurrences chemotherapy is administered to high risk patients. However, as few as 10-20% patients genuinely benefit because the clinical course for individuals with CRC remains difficult to predict, largely due to prognostically heterogeneous groups within same-stage tumour categories.
Inhibition of DNA methylation does not overcome docetaxel resistance in human breast cancer cells content piece image
Poster

Inhibition of DNA methylation does not overcome docetaxel resistance in human breast cancer cells

DNA mehtylation can lead to chemotherapy resistance in cancer. The aim of this study was to investigate the role of DNA methylation in docetaxel-resistant human breast cancer cells. Whereas the DNA methylation machinery is altered in docetaxel-resistant human breast cancer cells compared to docetaxel-sensitive human breast cancer cells, resistance to docetaxel could not be reversed using the DNA methylation inhibitor decitabine.
App Note / Case Study

Evaluation of PARP Inhibitors: Performed on BMG LABTECH’s FLUOstar Omega

Poly (ADP-ribose) polymerases (PARP) transfer ADP-ribose to itself and other nuclear proteins such as histones, therefore PARP play a crucial role in regulating DNA repair. A luminescent cellular PARP assay and a fluorescent cell viability assay were performed on BMG LABTECH’s FLUOstar Omega to evaluate PARP inhibitors. PARP inhibition has been demonstrated to potentiate the cytotoxicity of anti-cancer drugs and ionising radiation. The ability of the FLUOstar Omega to measure absorbance, lumines
Automation of cell proliferation assay sulforhodamine B fot the screening of stilbene derivatives content piece image
Poster

Automation of cell proliferation assay sulforhodamine B fot the screening of stilbene derivatives

The SRB assay was implemented on a TECAN Genesis workstation by optimizing cell numbers and liquid handling parameters. Anticancer drugs vinblastine, doxorubicin and 5-fluorouracil were used for standardizing the assay with a DU-145 cell line. To screen the antiproliferative activities of a series of stilbene derivatives, altogether 121 different stilbene structures, a plate layout was designed on 96-well format.
3D-QSAR Common Feature Pharmacophore Model for Polyphenols as Potential Anti-Malarial Agents content piece image
Poster

3D-QSAR Common Feature Pharmacophore Model for Polyphenols as Potential Anti-Malarial Agents

3D QSAR studies have been carried out on a series of polyphenols for their antimalarial activity using CATALYST program. Hypothesis with three features namely hydrophobic (1), hydrogen bond donor (1) and hydrogen bond acceptor (1) was found to the best, which mapped well with the most active and least active compound of the test set. This model can be used to develop drugs for malarial chemotherapy.
A genome-wide loss of function screen identifies new genes required for lung cancer cell proliferation content piece image
Poster

A genome-wide loss of function screen identifies new genes required for lung cancer cell proliferation

RNA interference (RNAi) provides an experimental tool for functional genomics analysis. We screened a genome-wide RNAi library to identify new genes involved in lung cancer cell proliferation. 72 % from the 257 genes identified were involved in general gene expression processes, 16 % were of unknown function or unrelated to proliferation, and 12% consisted of uncharacterized genes. These last sets of genes provide potential novel targets for lung cancer treatment.
Proteomic Profiling in Defining Chemoresistant Breast Cancer content piece image
Poster

Proteomic Profiling in Defining Chemoresistant Breast Cancer

This study aims to identify protein profiles in breast cancer cells as predictors of chemoresistance by using two-dimensional gel electrophoresis and MALDI-TOF peptide mass fingerprinting. Our findings provide further insights into the complex mechanisms of chemoresistance, as well as representing an attractive starting point for the identification of potential protein biomarkers to predict response to chemotherapy in breast cancer in vivo.
Usage of Low-Density Oligonucleotide Microarrays for Prognosis Prediction of Colorectal Cancer Patients content piece image
Poster

Usage of Low-Density Oligonucleotide Microarrays for Prognosis Prediction of Colorectal Cancer Patients

This study aimed to find individual up/down-regulated genes associated with progression and metastatic potential of colorectal cancers using low-density oligonucleotide microarrays spotted with genes known to be involved in process of metastasis development. We suppose that focusing on a particular biological pathway may be more useful than genome-wide screening for our purposes.
High Throughput Cell Cycle Analysis using Microplate Cytometry content piece image
Poster

High Throughput Cell Cycle Analysis using Microplate Cytometry

To improve screening efficiency, we have developed a cell cycle analysis method that uses an Acumen Explorer fluorescence microplate cytometer to measure the DNA content of propidium iodide stained fixed cells in microplates. We demonstrate that paclitaxel and vinblastine arrested CHO cells in the expected phase of the cell cycle.
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