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Flow Cytometry – Multimedia

Development of a Novel Xeno-free Medium for Feeder-free Culture of Human Stem Cells content piece image
Poster

Development of a Novel Xeno-free Medium for Feeder-free Culture of Human Stem Cells

A new xeno-free medium (ReproXFTM) has been developed for feeder-free culture of stem cells.
Cas9 Driven by an Optimal Promoter Improves Gene Editing in Eukaryotic Cell Lines when Paired with Synthetic crRNA and tracrRNA content piece image
Poster

Cas9 Driven by an Optimal Promoter Improves Gene Editing in Eukaryotic Cell Lines when Paired with Synthetic crRNA and tracrRNA

Presented here are results on the efficiency of using synthetic crRNA and tracrRNA to introduce gene editing events when co-transfected with a plasmid expressing Cas9. We explored the use of antibiotic and fluorescence activated cell sorting (FACS) methods for enrichment of cells that have undergone gene editing, and the use of multiple promoters to increase efficiency of gene editing with Cas9 and synthetic tracrRNA and crRNAs.
Cell-Mediated Cytotoxicity Assay by High-Throughput Direct Cell Counting in Microplates using Fluorescence-Based Image Cytometry content piece image
Poster

Cell-Mediated Cytotoxicity Assay by High-Throughput Direct Cell Counting in Microplates using Fluorescence-Based Image Cytometry

In this work, we demonstrate a novel high throughput cytotoxicity detection assay using the Celigo Imaging Cytometry method. Utilizing imaging cytometry, direct cell counting of live fluorescent target cells can be performed, which is a direct method for assessment of cytotoxicity.
Microbial Regulation of IgE Production in Early Life content piece image
Poster

Microbial Regulation of IgE Production in Early Life

Changes in gastrointestinal microbiota have been suggested to drive the increasing prevalence of food allergy. Our aim was to determine the impact of mouse and human intestinal microbiota on modulation of allergic parameters including IgE.
Video

Novel Technologies for Studying Functional Genomics at the Level of Single Cells

Higher eukaryotes, including plants, comprise tissues and organs comprising complex interspersions of different cell types.
Non-disruptively Count and Quantify Fluorescent iPS Colonies During Secondary Reprogramming: 7 min per 6-well Plate, Dual-fluorescence Whole Well Imaging Cytometry content piece image
Poster

Non-disruptively Count and Quantify Fluorescent iPS Colonies During Secondary Reprogramming: 7 min per 6-well Plate, Dual-fluorescence Whole Well Imaging Cytometry

Nexcelom’s Celigo imaging cytometer has been applied to provide automated, rapid assessment of iPS reprogramming.
MAB Discovery Technology: A Smart Way to Highly Diverse and Functional Therapeutic Antibodies content piece image
Poster

MAB Discovery Technology: A Smart Way to Highly Diverse and Functional Therapeutic Antibodies

MAB Discovery GmbH developped a highly integrated process which provides diverse antibodies by starting with a high number of B cells and filtering the relevant antibodies by an early-on functional screening.
Multi-Color Fluorescence Microscopy Application using Novel Brilliant Violet™ and Alexa Fluor® 594 Conjugated Monoclonal Antibodies content piece image
Poster

Multi-Color Fluorescence Microscopy Application using Novel Brilliant Violet™ and Alexa Fluor® 594 Conjugated Monoclonal Antibodies

Development of valuable new tools for the immunologist, to resolve multiple antigens simultaneously with fluorescence microscopy: Brilliant Violet 421™, Alexa Fluor® 488, Alexa Fluor® 594 and Alexa Fluor® 647 conjugated monoclonal antibodies.
New LEGENDplex™ Multi-Analyte Flow Assay Panels for Simultaneous Quantification of 13 Th Cytokines in Human and Mouse Samples content piece image
Poster

New LEGENDplex™ Multi-Analyte Flow Assay Panels for Simultaneous Quantification of 13 Th Cytokines in Human and Mouse Samples

We have developed two multiplexed assay panels, human and mouse, using fluorescence–encoded beads, suitable for use on various flow cytometers. Both panels allow simultaneous quantification of 13 human or mouse cytokines, including interleukins (IL-2, 4, 5, 6, 9, 10, 13, 17A, 17F, 21, 22), IFN-? and TNF-a. These Th cytokine panels can be used for serum, plasma, cell culture supernatant and other sample types, offering useful tools for biomedical research and drug discovery.
Discrimination of spectrally overlapping fluorochromes using Sony Corporation’s (SP6800) Spectral Analyzer content piece image
Poster

Discrimination of spectrally overlapping fluorochromes using Sony Corporation’s (SP6800) Spectral Analyzer

SP6800, a novel spectral analyzer from Sony Corporation, uses an innovative optics system that collects all emitted light and eliminates the need for band pass filters. Our data show that the SP6800 can perform all the analysis similar to a traditional cytometer with the added feature of separating spectrally overlapping fluorophores.
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