This page provides educational resources, events, news and products related to western blot techniques and applications, including assay optimization, detection techniques and reproducibility.
Western Blot – Highlights
Western Blot Procedures, Analysis and Purpose
There are many reasons you may need to determine the presence (or absence) and size of a given protein within a sample, potentially against a background of many others. The western blot is one such technique that offers these capabilities. In this article, we discuss what western blots are, how they are performed, what they show us and how they differ to related techniques.
How To Guide
How To Validate Your Antibodies
Download this guide to learn the importance of context in optimization, why controls can make or break an assay and how sample status may be confirmed in an antibody-independent manner.
Overcoming Western Blot Reproducibility Problems: Tips for Producing More Accurate and Reliable Data
This article outlines key workflow steps to help researchers perform better western blots. By executing best practices in these steps – sample preparation, gel electrophoresis, gel transfer, protein detection, and image analysis – researchers can expect good western blot data.
An Automated Age for Western Blotting
Western blotting has been a core technique in molecular biology for many years, however, optimization and reproducibility can be problematic. We spoke to Dr Alexandre Lucas about the automation of western blotting and the difference this advance is making to research.
How To Guide
How To Choose the Right Western Blot Detection Method
It is important to understand the advantages and disadvantages of different ways of detecting a protein of interest on a western blot membrane. Here, we consider the three main types of protein detection for western blot; radioactive, enzymatic, and fluorescent.
Improving Image Integrity in Scientific Papers
This article sheds light on the issue of image integrity in academic publishing and gives advice on how to reduce the risk.
Unlocking the Power of Spatial Biology
In this interview, we speak to Vikram Devgan at NanoString Technologies to learn more about how added context from spatial biology techniques can provide more detailed insights during tissue analysis.
Principal Tools Uncovering Complexities of Novel Drug Target Discovery
In this article, Dr. Kenneth Oh discusses how a comprehensive qPCR–western blot–multiplex assay workflow can revolutionize drug discovery approaches.
Evolution of Lyme Disease Testing and Future Perspectives
This article explores the current state of Lyme disease testing and how improvements could be made in the future to improve time to diagnosis.
App Note / Case Study
Fast and Accurate Protein Quantitation
Protein quantitation is an integral part of many protein biology workflows and a necessary step before commonly used techniques such as protein electrophoresis, western blotting, mass spectrometry and immunoassays.
Biotium Introduces New Line of Antibodies for Exosome Detection
Biotium has announced the release of new ExoBrite Antibody Conjugates. These antibodies were curated specifically for optimal detection of exosome markers CD9, CD63 and CD81 in isolated exosomes or exosome extracts with little to no background.