Fully-Automated Enzyme Optimal pH Analysis
pH is a critical parameter in method development and routine enzyme assay measurement. At the ideal pH value, called optimal pH, enzymes have their highest activity. Accurate and reliable determination of the optimal pH is important for successful enzyme assay method development.
Get Smart with Protein Digestion
Is your protein digestion protocol long, laborious and irreproducible? Discover how Thermo Scientific™ SMART Digest™ Kits provide faster, simpler and more reproducible peptide mapping and bioanalysis workflows for the biopharmaceutical industry.
Behind the Science, ep. 12: My Samples Are Too Dirty For MS
Today our host Jen Fournier is in the lab with the dirtiest, grimiest samples. She knows if she tries to run them on LC-MS as-is, she’d degrade the column, get low sensitivity … and have to clean the mass spectrometer. Is there a better way to start with clean samples?
Detection and Characterization of Nanoparticles in Food and Biological Materials
With the increasing use of nanotechnology in food and consumer products, there is a need for reliable detection and characterization methods for nanoparticles (NPs) in complex matrices. NPs often interact with each other or with their surroundings leading to aggregation, adhesion to surfaces or dissolution in dispersion solvents. Accurate and precise characterization of metrics such as size, shape, particle mass and number concentration therefore remains a challenging analytical task. Asymmetric flow field flow fractionation (AF4) hyphenated with inductively coupled plasma mass spectrometry (ICP-MS) is a powerful technique for the detection and characterization of inorganic NPs in complex matrices. In order to acquire accurate data the AF4 separation method and settings must be optimized for each new sample matrix and analyte NP combination. Furthermore, additional information obtained by single particle (sp) ICP-MS analysis of collected fractions or an imaging method such as transmission electron microscopy (TEM) are necessary for trouble shooting and for independent verification of results. spICP-MS as stand-alone technique is a powerful screening method for many inorganic NPs. A number of examples where AF4-ICP-MS and single particle ICP-MS were applied for the detection and characterization of NPs in food and biological matrices will be presented including: • Silver NPs in chicken meat • Silicon dioxide NPs in tomato soup • Aluminum-containing NPs in noodles • Gold NPs in cells
LabTube Meets Shimadzu at Pittcon 2016
LabTube Meets Dan Davis, Elemental Spectroscopy Product Manager, from Shimadzu at Pittcon 2016, Atlanta.
Automated LC-MSMS methods for Clinical Diagnostics - Realized for therapeutic drug monitoring and vi
Tecan and RECIPE have worked together to automate ClinMass® LC-MSMS Complete Kits for the analysis of vitamin D and immunosuppressants on a Freedom EVO® platform, to help meet the demand for high throughput sample preparation for mass spectrometry in clinical labs.
Quantitative Top-Down Proteomics & Its Impact on Clinical Research/Basic Biology
Listen as Prof Neil Kelleher, discusses how he believes the Orbitrap Fusion Lumos MS might better characterize unexpected PTMs in Top-Down Proteomics to decode cellular function.
Beyond Genomics For Personalized Medicine
The proteome is the most functional component encoded for in the genome. Advances in proteomic technologies allow comprehensive profiling of of signaling pathways, precise expression of therapeutic targets in tissues and cellular subcompartments, and of metabolomic features that determine likelihood of therapeutic response. Moreover the current paradigm shift in cancer therapy with dramatic and durable responses to antibody and checkpoint therapeutics that trascend tumor genomic profiles further emphasize opportunities to identify novel targets and tailor treatment based on their expression patterns. The potential for personalizing cancer treatment based on proteomic and metabolomic profiles will be presented for epithelial type tumors including lung and pancreatic cancer.
Microinjecting CRISPR: Options and key considerations for creating transgenic animal models
CRISPR is revolutionizing the life sciences. Genome editing using RNA-programmable nucleases opens up a wealth of experimental options. At the same time, it makes creating new cell and animal models faster, easier and cheaper than in the past. Consequently, researchers in areas as diverse as cell/developmental biology, genetics or clinical research are turning to CRISPR as their method of choice.
Horizon Discovery: The Power of Personalized Medicine
Learn about how Personalized Medicine is changing how drugs are being developed and how healthcare is being delivered, and how Horizon is helping to enable this change.
Loss-of-Function Genetic Screens with RNAi and CRISPR Libraries
Loss-of-function genetic screens with pooled sgRNA and shRNA libraries aid in discovery / prioritization of drug targets, understanding of disease progression and gene-disease associations, analysis of signal transduction pathways, characterization of the mechanisms for compounds, uncovering of synergistic gene interactions, and identification of markers of drug resistance and sensitivity. This webinar presents a comparison of loss-of-function genetic screens performed with CRISPR versus RNAi technology.