A Microfluidic Chip based Model for the Study of Full Thickness Human Intestinal Tissue

Video   Oct 18, 2016

 

The dual-flow device is designed to mimic the human body and maintain tissue in a functional state ex-vivo. This can be used for the investigation into diseases such as inflammatory bowel disease (IBD) including Crohns disease and Ulcerative Colitis. As the causes of IBD remain unknown many patients with extensive disease may develop complications that are potentially life-threatening, including an increased risk of colorectal cancer. The device has been characterised to ensure the tissue remains viable for up to 72 hours. Tissue has been investigated through measuring cell death via LDH release, cell proliferation via Ki-67 staining and MTS, and also through histology by H&E and PAS staining. IBD is thought to be caused by an influx of luminal content into the mucus layer of the gut stimulating an inflammatory response. Investigations using biopsies with an intact layer of mucus have shown that inflammatory markers (e.g. IL-10, IL-12 and calprotectin) are detectable in the effluent. The device provides a platform that allows the complex interaction between the host, mucus production and the commensal bacteria to be studied; providing a same species model for investigation of IBD. It is simple to use and is easily adopted by other research laboratories.

 
 
 
 

Recommended Videos

LC-MS/MS for Bioanalytical Peptide and Protein Quantification: MS Considerations

Video

Caitlin Dunning, Associate Scientist, discusses how to use mass spectrometry to develop sensitive, selective, and robust quantification methods for peptide and protein quantification, including: basic mass spectrometry considerations, MS tuning, and best practices for success.

WATCH NOW

Now you can visualize and analyze complex 3D cell models – with Harmony 4.8 software

Video

See what you’re missing with the new capabilities of Harmony® 4.8 software for imaging, analysis, and visualization of complex 3D cell models.

WATCH NOW

Contrasting LBAs and LC-MS for Peptide and Protein Bioanalysis

Video

Kelly Doering discusses ligand binding assays (LBAs) and compares the advantages and limitations of this technology to LC-MS.

WATCH NOW

 

Comments | 0 ADD COMMENT

Like what you just watched? You can find similar content on the communities below.

Drug Discovery

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE

We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy