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Best Practices for 3D Cell Imaging With Dr. Ann Rossi Bilodeau
Video

Best Practices for 3D Cell Imaging With Dr. Ann Rossi Bilodeau

Best Practices for 3D Cell Imaging With Dr. Ann Rossi Bilodeau
Video

Best Practices for 3D Cell Imaging With Dr. Ann Rossi Bilodeau

Despite the growing relevance of 3D cell culture as a research model, imaging techniques used to characterize these models are highly limited. Due to the thickness and opacity of 3D cellular structures, most current imaging technologies cannot penetrate to the center of the tissues, resulting in only the outer two or three layers of cells being detected. This causes the dark centers that are often seen in images of 3D cell culture models. This proves highly problematic for accurate analysis as these outer cells are most exposed to compounds, nutrients and oxygen and thus do not reflect the entire cell population.

In this installment of Teach Me in 10 we speak with Dr. Ann Rossi Bilodeau Senior Bioprocess Applications Scientist at Corning Life Sciences about the best practices for 3D cell imaging, whether tissue clearing reagents can be used to clear spheroids expressing fluorescent proteins and we learn more about the new 3D Clear Tissue Clearing reagent from Corning Life Sciences.

Fore more Teach Me in 10 videos, make sure you visit our hub page.


Meet The Author
Lucy Lawrence
Lucy Lawrence
Scientific Content Producer
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