Can We Trust Western Blots?

Video   Mar 28, 2017

 

Aldrin Gomes, Associate Professor of Neurobiology at UC Davis, talks about key success factors to instill trust in western blotting data. Western blotting is a widely used technique to identify and quantify proteins in complex samples. Unfortunately, as an editor of several journals, Prof. Gomes routinely observes Western blots that are not properly conducted. The generally low quality of western blotting data has led scientists to question whether we can trust protein quantification when using western blots. Reducing errors and misinterpretations requires standardized procedure and proper controls. In this webinar, Prof. Gomes discusses the following key factors for high-confidence western blot data:

1) Quality of the primary and secondary antibodies and the blocking reagent

2) Proper target protein controls 3) Choice of total protein loading control vs. housekeeping protein loading control to normalize the target protein measurement.

Prof. Gomes also presents tips to reduce the overall time required for first-rate western blots and how to minimize the pain of going through a long procedure. The scientific community needs to continue to increase the awareness of problems associated with western blotting and introduce, improve, and standardize basic criteria for western blotting practices so that the most investigators will be successful in eliminating common problems associated with “bad” western blots.

 
More Information
 
 
 

Recommended Videos

2017 ALZHEIMER'S DISEASE FACTS AND FIGURES

Video

Video describing the prevalence of Alzheimer's Disease from the Alzheimer's Association

WATCH NOW

Behind the Science, S2 Ep6: Dimple Shah on detecting phthalates in whisky

Video

Got phthalates? We hope not. Jen talks with senior scientist Dimple Shah about how these potential endocrine disruptors are found in food and beverages such as distilled beverages and whisky

WATCH NOW

How Dangerous Are Concussions?

Video

Each year in the United States, players of sports and recreational activities receive between 2.5 and 4 million concussions. How dangerous are all those concussions?

WATCH NOW

 

Comments | 0 ADD COMMENT

Like what you just watched? You can find similar content on the communities below.

Analysis & Separations Proteomics & Metabolomics Neuroscience

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE