Continuous Live Cell Analysis at Sygnature Discovery
Video Feb 08, 2017
In July 2015, Sygnature and Essen BioScience Ltd., a world leader in the field of cell-based in vitro assays and instrumentation, announced that they had established a strategic alliance to incorporate Essen’s unique phenotypic assay, live-cell imaging and ion channel technologies into Sygnature’s integrated drug discovery projects. This alliance has been very productive and has also resulted in the addition of the IncuCyte® ZOOM System by Sygnature into its in-house capabilities. IncuCyte® ZOOM offers plate-based continuous cell imaging within a controlled tissue-culture environment, allowing real time quantitative live-cell, non-invasive analysis. Assaying cellular systems using live-cell imaging affords an array of advantages when compared to classical single end-point functional cellular assay read-outs; these include, label-free analysis, no cell disruption during analysis, multiplexing and kinetic data generation. The adoption of the IncuCyte® ZOOM System has enhanced Sygnature’s research expertise across a broad range of therapeutic areas including oncology, immunology, inflammation and neuroscience.
Behind the Science, S2-Ep1: Classic rock & HPLC with Pat McDonaldVideo
Every good rock band starts in the basement. So that's where we're heading ... to the basement of Waters to see the first chromatography systems! Pat McDonald, corporate fellow emeritus at Waters, tells us about Jim Waters and Waters Associates' inventions.
The first commercial "high pressure" chromatography system for polymer chemists, the GPC-100, from 1963. The ALC-100, developed in late 1970 for separating isomers in the synthesis of B12. The M6000 pump that came in 1972, followed by the U6K injector, the first commercial "small" particle columns (10 µm!). Bringing those pieces into an assembly led to the first modern "high performance" liquid chromatography instrument, now known as HPLC.
Read up on the history of Jim Waters and his chromatography people: http://bit.ly/2mwT2BI
Electrophysiology Patch-Clamp Equipment: The Very Basics (Part 1)Video
Michael Nestor gives you a simplistic overview of what equipment you might need or encounter when setting up a single-cell patch clamp rig for whole-cell patch and electrophysiological recordings from neurons.WATCH NOW