Development of a Point-of-Care Diagnostic for Influenza Detection with Antiviral Treatment Effectiveness Indication

Video   Jun 23, 2015

 

Currently, diagnosis of influenza is performed either through either polymerase chain reaction (PCR) or through rapid antigen detection assays. The rapid antigen detection assays currently available are highly specific but not very sensitive, and lack the ability to show if the strain of influenza detected is susceptible to antiviral agents, such as Tamiflu and Relenza. The ability to rapidly determine if a patient has an infectious disease and what type of treatment the infection will respond to, would significantly reduce the treatment decision time, shorten the impact of symptoms, and minimize transfer to others. In this study, a point-of-care style ┬ÁPAD (microfluidic paper-based diagnostic) for influenza has been developed with the ability to determine antiviral susceptibility of the strain for treatment decision. The assay exploits the enzymatic activity of surface proteins present on all influenza strains, and potential false positive responses can be mitigated. A simple nasal swab sample diluted in water is added to the device, the sample is distributed to 4 different reagent zones, and development of the enzymatic substrate under different buffer conditions takes place on bottom of the device. Analysis can be performed by eye or through a colorimetric image analysis smartphone app.

 
 
 
 

Recommended Videos

Professor Anita Maguire Tells Us About How and Why Her Lab Performs Chemistry in Flow

Video

Lab Tube Meets Prof. Anita Macguire at Flow Chem Europe 2017. Anita describes how and why her lab performs their organic chemistry reactions in Flow.

WATCH NOW

Catherine Goh Discusses the Benefits of SiPOOLs Developed by SiTools Biotech GmbH

Video

LabTube Meets Catherine Goh, Head of Product Development and Marketing, SiTools Biotech GmbH.

WATCH NOW

The Evolution of PCR

Video

Launching the first FDA-approved PCR test in 1993, Roche Molecular Diagnostics has not only defined and refined PCR, but it has remained the clear leader of this technology.

WATCH NOW

 

Comments | 0 ADD COMMENT

Like what you just watched? You can find similar content on the communities below.

Diagnostics Drug Discovery Informatics & Automation Proteomics & Metabolomics

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE