We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

Advertisement

Diagnosis of Disorders Affecting Steroid Synthesis and Metabolism by Mass Spectrometry

Video   Mar 03, 2015

 

The approach that has been employed for the characterization of almost all disorders of steroid hormone biosynthesis and metabolism is not based on determining the circulating concentration of the hormones, but on the study of the urinary steroid profile. The rationale behind this approach is that most hormonal imbalances caused by enzyme deficiencies or “blocks” lead to the depletion of a steroid product and to the build-up of its upstream precursors. Thus, a ratio of urinary metabolites of the precursor to urinary metabolites of the product should indicate if there is such a block. The ranges for such ratios in normal and affected individuals for most steroid disorders have been traditionally established by GC-MS. Nowadays, this technique is being replaced by superior LC-MS/MS methodologies which allow the simultaneous identification and quantification of both major and minor metabolites.

 
 
 
Advertisement
 

Recommended Videos

Making of Neoma MC-ICP-MS

Video

For scientists whose research requires high-precision isotope ratio data in fields of cosmo- and geochemistry, geochronology and biology, nuclear safeguards, forensics, metalomics, and provenance studies, we have created a new multicollector ICP-MS. Designed and developed in Bremen, building on the strengths of our multi-collector platform with field-proven technologies such as RPQ, 10^13 ohm Amplifier Technology and Jet Interface.

WATCH NOW

COVID, One Year Later: What Do We Know Now?

Video

ACS Reactions catch up with experts they talked to at the start of the pandemic and ask, "what do we know now that we didn’t back then?"

WATCH NOW

Detecting Psychoactive Substances in Forensic Toxicology

Video

This instalment of Teach Me in 10 features Dr Lorna Nisbet, a senior lecturer at Anglia Ruskin University. Lorna’s research interests are within the field of forensic toxicology where she specializes in the detection of new psychoactive substances that have been chemically altered to produce new types of high.

WATCH NOW

 

Like what you just watched? You can find similar content on the communities below.

Analysis & Separations Diagnostics Proteomics & Metabolomics

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE