Diagnosis of Disorders Affecting Steroid Synthesis and Metabolism by Mass Spectrometry

Video   Mar 03, 2015

 

The approach that has been employed for the characterization of almost all disorders of steroid hormone biosynthesis and metabolism is not based on determining the circulating concentration of the hormones, but on the study of the urinary steroid profile. The rationale behind this approach is that most hormonal imbalances caused by enzyme deficiencies or “blocks” lead to the depletion of a steroid product and to the build-up of its upstream precursors. Thus, a ratio of urinary metabolites of the precursor to urinary metabolites of the product should indicate if there is such a block. The ranges for such ratios in normal and affected individuals for most steroid disorders have been traditionally established by GC-MS. Nowadays, this technique is being replaced by superior LC-MS/MS methodologies which allow the simultaneous identification and quantification of both major and minor metabolites.

 
 
 
 

Recommended Videos

Behind the Science, S2 Ep8: Dimple Shah on how to tell if rice is organic

Video

Jen talks with Dimple Shah about how analytical science is used and the sensitivity of LC-MS/MS required to detect pesticide residues in rice, and unexpected detection of residues in an organic rice sample.

WATCH NOW

Scientist Feedback on the NanoDrop One Microvolume UV-Vis Spectrophotometer

Video

Watch this testimonial video with a University of Cambridge scientist who studies the chemical biology of nucleic acids.

WATCH NOW

LabTube TV meets Elizabeth Admirand, Epizyme

Video

Elizabeth Admirand, Principal Research Scientist, discusses how entering the clinical stage drove changes to Epizyme's sample management systems for compounds and biologics.

WATCH NOW

 

Comments | 0 ADD COMMENT

Like what you just watched? You can find similar content on the communities below.

Analysis & Separations Diagnostics Informatics & Automation Proteomics & Metabolomics

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE