We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

Diagnosis of Disorders Affecting Steroid Synthesis and Metabolism by Mass Spectrometry

Video   Mar 03, 2015

 

The approach that has been employed for the characterization of almost all disorders of steroid hormone biosynthesis and metabolism is not based on determining the circulating concentration of the hormones, but on the study of the urinary steroid profile. The rationale behind this approach is that most hormonal imbalances caused by enzyme deficiencies or “blocks” lead to the depletion of a steroid product and to the build-up of its upstream precursors. Thus, a ratio of urinary metabolites of the precursor to urinary metabolites of the product should indicate if there is such a block. The ranges for such ratios in normal and affected individuals for most steroid disorders have been traditionally established by GC-MS. Nowadays, this technique is being replaced by superior LC-MS/MS methodologies which allow the simultaneous identification and quantification of both major and minor metabolites.

 
 
 
 

Recommended Videos

Cas13: Cas9's Cool Detective Cousin

Video

This animation depicts how Cas13—a CRISPR-associated protein—may be adapted to detect human disease. This new diagnostic tool, called SHERLOCK, targets RNA (rather than DNA), and has the potential to transform research and global public health.

WATCH NOW

How Do Pregnancy Tests Work?

Video

Over-the-counter pregnancy tests give potentially life-changing results with a pretty high rate of accuracy. But how do they work? Watch this video to find out.

WATCH NOW

How Do Scientists Know if Plastic Containers are BPA-free? | Behind the Science, S3 Ep5

Video

How do consumer packaging companies test their products to maintain "BPA-free" label claims? VICAM, a division of Waters that develops rapid diagnostics and laboratory sample prep technologies for testing quality and safety in food and agriculture, has a new fit-for-purpose BPA test that pairs immunoaffinity chromatography to extract and elute BPA for subsequent measurement by LC-MS/MS.

WATCH NOW

 

Like what you just watched? You can find similar content on the communities below.

Analysis & Separations Diagnostics Proteomics & Metabolomics

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE