Diagnosis of Disorders Affecting Steroid Synthesis and Metabolism by Mass Spectrometry

Video   Mar 03, 2015

 

The approach that has been employed for the characterization of almost all disorders of steroid hormone biosynthesis and metabolism is not based on determining the circulating concentration of the hormones, but on the study of the urinary steroid profile. The rationale behind this approach is that most hormonal imbalances caused by enzyme deficiencies or “blocks” lead to the depletion of a steroid product and to the build-up of its upstream precursors. Thus, a ratio of urinary metabolites of the precursor to urinary metabolites of the product should indicate if there is such a block. The ranges for such ratios in normal and affected individuals for most steroid disorders have been traditionally established by GC-MS. Nowadays, this technique is being replaced by superior LC-MS/MS methodologies which allow the simultaneous identification and quantification of both major and minor metabolites.

 
 
 
 

Recommended Videos

Behind the Science, S2 Ep6: Dimple Shah on detecting phthalates in whisky

Video

Got phthalates? We hope not. Jen talks with senior scientist Dimple Shah about how these potential endocrine disruptors are found in food and beverages such as distilled beverages and whisky

WATCH NOW

DeNovix DS-11 FX Spectrophotometer / Fluorometer Series: 1uL UV-Vis + Fluorescence

Video

The DeNovix DS-11 FX Series of Spectrophotometers / Fluorometers combine UV-Vis absorbance and fluorescence methods in one compact , maintenance-free instrument. Rapidly quantify nucleic acids and protein samples selecting your choice of UV-Vis or Fluorescent modes.

WATCH NOW

Talking Ion Chromatography for Pharmaceutical Analysis with Dr Jeffrey Rohrer

Video

Dr Jeff Rohrer, Director of Applications Development at Thermo Fisher Scientific provides experienced insights into the USP monograph modernization initiative, the role that ion chromatography may play in this and the current/future state of ion chromatography innovations.

WATCH NOW

 

Comments | 0 ADD COMMENT

Like what you just watched? You can find similar content on the communities below.

Analysis & Separations Diagnostics Informatics & Automation Proteomics & Metabolomics

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE