Diagnosis of Disorders Affecting Steroid Synthesis and Metabolism by Mass Spectrometry

Video   Mar 03, 2015

 

The approach that has been employed for the characterization of almost all disorders of steroid hormone biosynthesis and metabolism is not based on determining the circulating concentration of the hormones, but on the study of the urinary steroid profile. The rationale behind this approach is that most hormonal imbalances caused by enzyme deficiencies or “blocks” lead to the depletion of a steroid product and to the build-up of its upstream precursors. Thus, a ratio of urinary metabolites of the precursor to urinary metabolites of the product should indicate if there is such a block. The ranges for such ratios in normal and affected individuals for most steroid disorders have been traditionally established by GC-MS. Nowadays, this technique is being replaced by superior LC-MS/MS methodologies which allow the simultaneous identification and quantification of both major and minor metabolites.

 
 
 
 

Recommended Videos

Pill for Better Diagnosis of Breast Cancer

Video

Researchers from the University of Michigan have developed a diagnostic imaging pill enabling the early and more accurate diagnosis of cancerous tumors in breast tissue.

WATCH NOW

How 2D-LC is Used in Forensic Science at Boston University Medical School - Behind the Science

Video

The focus of a forensic toxicology laboratory is to determine the presence or absence of drugs in biological samples. Often times when it comes to drug testing, the sensitivity of the analytical method is critical because scientists need to detect chemical compounds in very small amounts. Multidimensional liquid chromatography (2DLC) is one option forensics labs can use to test biological samples, such as urine, blood, oral fluid, hair, to determine if an illicit drug or toxin played a role in a person's death.

WATCH NOW

Exploring Multiple Dimensions in the Forensics of “Space Candy” with Boston Uni. - Behind the Science

Video

In forensic toxicology laboratories, the accuracy and precision of an analytical technique are essential in determining what drugs or chemicals may have played a role in an individual's death. Is it possible to use one analytical technique to test a variety of different matrices - liquid or solid?

WATCH NOW

 

Like what you just watched? You can find similar content on the communities below.

Analysis & Separations Diagnostics Proteomics & Metabolomics

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE

We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy