We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

Imaging Gene Regulation in Living Cells at the Single Molecule Level

Video   Jan 03, 2017

 

Combinatorial cis-regulatory network in animal genomes represents the foundational gene expression mechanism for directing cell-fate commitment and maintenance of cell identity by transcription factors (TFs). However, it remains poorly understood how TFs dynamically search for specific target sites in the nucleus of a living cell and how the 3D spatial organization of the genome contributes to spatiotemporal gene expression programs during animal development. Here I will present our latest efforts in harnessing a set of advanced super-resolution, single-molecule imaging approaches to study how enhancer-binding pluripotency factors (Sox2/Oct4) dynamically search for and assemble on their cognate sites in live embryonic stem (ES) cells. Specifically, we developed a method capable of discriminating specific DNA binding events from non-specific macromolecular interactions in live cells; our single-molecule measurements also describe several key kinetic features (3D diffusion periods, number of trials, search times, residence times etc.) associated with in vivo TF target search dynamics and interestingly our data support an ordered Sox2-Oct4 assembly at endogenous loci. Finally, I will present an imaging strategy using the lattice light sheet microscope to probe nanometer-scale 3D enhancer organization in single live ES cells.

 
 
 
 

Recommended Videos

HPLC - Normal Phase vs Reverse Phase

Video

Basic info about Normal Phase and Reverse Phase HPLC.

WATCH NOW

A revolution in the world of X-Ray Diffraction

Video

With the 3rd generation Empyrean, Malvern Panalytical has now redefined the concept of a multipurpose diffractometer: the newly designed MultiCore Optics enable the largest variety of measurements without any manual intervention.

WATCH NOW

Attend the Isotope fingerprints e-learning and go on a journey with the Isotope Hunter

Video

In this e-learning series you will get an introduction to Isotope Fingerprints.

WATCH NOW

 

Like what you just watched? You can find similar content on the communities below.

Analysis & Separations Genomics Research

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE