We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

Immune Cell Killing of Tumor Cells | Automatically Measure the Time Course with the IncuCyte® System

Video   Oct 06, 2016

 

Time-lapse movies acquired using IncuCyte® ZOOM live-cell analysis system showing a T cell sub population of PBMCs, activated with anti-CD3 antibody and IL-2, killing target SK-OV-3 tumor cells. Labeling of the SK-OV-3 cancer cells with the NucLight Red™ Live-Cell Labeling reagent enables direct counting of viable tumor cell numbers over-time. Addition of the IncuCyte™ Caspase 3/7 apoptosis reagent enables simultaneous detection of cells undergoing apoptosis (green fluorescence).

Assay concept:

● Target tumor cells are co-cultured with your immune cells of choice (e.g., T cells or human peripheral blood mononuclear cells; PBMCs).
● Tumor cell death is measured directly and in real-time by adding the mix-and-read IncuCyte™ Caspase 3/7 Reagent to your cultures.
● Tumor cell counts can be enabled by labeling target cells with NucLight™ Live-Cell Labeling Reagents
● Automated image analysis enables selective quantitation of tumor cell death.

Learn more at www.essenbio.com/immune-cell-killing

 
Company Information
 
 
 

Recommended Videos

The Cell-based Human Proteome Project - Neil Kelleher - TEDxNorthwesternU

Video

Neil's TEDx Talk, A Plan to Weigh Every Protein in the Human Body: The Cell- Based Human Proteome Project presents a "big science" project with huge implications for how proteins in the human body are catalogued and used for drug therapies and treatments.

WATCH NOW

A Voice for Diversity in Science

Video

Only 17% of the English-language biographies on Wikipedia are about women – but the statistic won’t stay that low for long if Dr Jess Wade has her way. A passionate advocate for diversity in science, Jess balances her work as an award-winning physicist at Imperial College London with her role as a ‘Wikipedian’, creating and uploading the biographies of underrepresented groups in science.

WATCH NOW

Wireless Sensors for Neonates Remove the Cuddle-barrier Tangle

Video

A pair of soft, flexible wireless body sensors have been developed to monitor vital health indicators of neonatal babies. With the tangle of wires removed, parents and babies have more opportunity for skin-to-skin contact.

WATCH NOW

 

Like what you just watched? You can find similar content on the communities below.

Diagnostics Drug Discovery Immunology & Microbiology

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE