We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

Immune Cell Killing of Tumor Cells | Automatically Measure the Time Course with the IncuCyte® System

Video   Oct 06, 2016

 

Time-lapse movies acquired using IncuCyte® ZOOM live-cell analysis system showing a T cell sub population of PBMCs, activated with anti-CD3 antibody and IL-2, killing target SK-OV-3 tumor cells. Labeling of the SK-OV-3 cancer cells with the NucLight Red™ Live-Cell Labeling reagent enables direct counting of viable tumor cell numbers over-time. Addition of the IncuCyte™ Caspase 3/7 apoptosis reagent enables simultaneous detection of cells undergoing apoptosis (green fluorescence).

Assay concept:

● Target tumor cells are co-cultured with your immune cells of choice (e.g., T cells or human peripheral blood mononuclear cells; PBMCs).
● Tumor cell death is measured directly and in real-time by adding the mix-and-read IncuCyte™ Caspase 3/7 Reagent to your cultures.
● Tumor cell counts can be enabled by labeling target cells with NucLight™ Live-Cell Labeling Reagents
● Automated image analysis enables selective quantitation of tumor cell death.

Learn more at www.essenbio.com/immune-cell-killing

 
Company Information
 
 
 

Recommended Videos

Plug-and-play Paperfluidic Diagnostics

Video

Researchers at MIT have developed half-inch modular blocks that can be constructed to produced different diagnostic devices. They hope that their low-cost, easy-to-assemble half-inch creations will soon land in the hands of many small laboratories around the world, to improve infection detection and monitoring.

WATCH NOW

John Ioannidis on Moving Toward Truth in Scientific Research

Video

PLOS author John Ioannidis, Professor of Medicine, of Health Research and Policy, and of Statistics at Stanford University, considers how the scientific community can move toward greater truth in published research.

WATCH NOW

Tracking Cancer on the Move: Finding That One in a Million

Video

An analogy of finding CTCs: Imagine you have three friends, and they are somewhere in Canada. But you don't know where they are. Also, they change their clothes and hair colour every day.
Watch to learn about this liquid biopsy test that has successfully progressed from the research lab to the clinic.

WATCH NOW

 

Like what you just watched? You can find similar content on the communities below.

Diagnostics Drug Discovery Immunology & Microbiology

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE