Immune Cell Killing of Tumor Cells | Automatically Measure the Time Course with the IncuCyte® System

Video   Oct 06, 2016


Time-lapse movies acquired using IncuCyte® ZOOM live-cell analysis system showing a T cell sub population of PBMCs, activated with anti-CD3 antibody and IL-2, killing target SK-OV-3 tumor cells. Labeling of the SK-OV-3 cancer cells with the NucLight Red™ Live-Cell Labeling reagent enables direct counting of viable tumor cell numbers over-time. Addition of the IncuCyte™ Caspase 3/7 apoptosis reagent enables simultaneous detection of cells undergoing apoptosis (green fluorescence).

Assay concept:

● Target tumor cells are co-cultured with your immune cells of choice (e.g., T cells or human peripheral blood mononuclear cells; PBMCs).
● Tumor cell death is measured directly and in real-time by adding the mix-and-read IncuCyte™ Caspase 3/7 Reagent to your cultures.
● Tumor cell counts can be enabled by labeling target cells with NucLight™ Live-Cell Labeling Reagents
● Automated image analysis enables selective quantitation of tumor cell death.

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