We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

Advertisement

Innovative Methods to Detect Viruses in Food

Video   Oct 06, 2016

 

Because of this increasing number of enteric virus outbreaks, it has become even more important to have reliable and widely applicable techniques for the detection and quantification of viruses in food samples. Moreover, since the infectious dose of enteric viruses is very low, sensitive methods are therefore needed when screening food products for viral pathogens. So far, the current ‘gold standard’ for detection of enteric viruses is quantitative reverse transcription PCR (RT-qPCR) but this method cannot discriminate between infectious and inactivated viruses.The relationship between the number of infectious viruses and the number of genome copies detected by RT-qPCR is not a constant, mainly in food samples, since this ratio may vary depending on environmental conditions.A promising new strategy to assess viral infectivity relies on the use of nucleic acid intercalating dyes, or viability dyes, such as ethidium monoazide (EMA) or propidium monoazide (PMA) as a sample pretreatment before applying molecular techniques. Theoretically, these compounds cannot penetrate intact capsids but are able to penetrate damaged or destroyed capsids. Once penetrated, the dye intercalates covalently into RNA after exposure to strong visible light, interfering with PCR amplification. Until now, viability dyes combined with RT-qPCR have successfully been applied to virus suspensions in order to discriminate between infectious and inactivated viruses. However, the effectiveness of viability dyes to discriminate between infectious and inactivated viruses in food samples has scarcely been explored.

 
Company Information

Request Information

 
 
Advertisement
 

Recommended Videos

Meet the Isotope Hunters at Eurofins (Nantes)

Video

The Isotope Hunters at Eurofins (Nantes) investigate, also with isotopes, on food authenticity, to ensure consumer trust and fairer market competition. No matter the samples, wine, juice, honey, they are looking to identify adulteration and fraud in food and beverage.

WATCH NOW

Cleaning the Chemical Industry With a Hammer

Video

Throughout many years working in labs, the shocking impact of chemical enterprise on the environment has led chemist Thomas-Xavier Métro to explore the wonderful ways in which organic chemists can reduce their use of solvents, and help to protect our natural world. His talk hopes to show you how.

WATCH NOW

Introduction to ICP-MS – Part II

Video

In part two of their ICP-MS Teach Me in 10, Abe Gutierrez and Ed McCurdy help to answer the question: Why do we need triple quadrupole ICP-MS?

WATCH NOW

 

Like what you just watched? You can find similar content on the communities below.

Applied Sciences Genomics Research

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE