We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

Advertisement

Knowledge-Based Identification of Plant Stress Regulators

Video   Jan 19, 2015

 

Here we adapt digital droplet PCR to operate under COLD-PCR conditions, thereby preferentially amplifying mutation-containing sequences in droplets.  COLD-PCR enables amplicons with mutations at any position to be amplified preferentially, while wild-type samples are preferentially suppressed. The preferentially amplified sequences can subsequently be sequenced. We validate the merging of technologies in serially diluted TP53 and KRAS mutated sequences. By merging COLD-PCR and digital -PCR technologies we enable digital mutation scanning, a new frontier for digital-PCR. A single reaction can now interrogate numerous mutations, and replaces multiple individual digital-PCR reactions.

 
 
 
Advertisement
 

Recommended Videos

Using Deep Learning To Detect Cancerous Skin Lesions

Video

Researchers at the Wyss Institute and MIT have developed a deep learning algorithm that can differentiate suspicious pigmented lesions from non-cancerous skin marks in wide-field images of patients' skin.

WATCH NOW

Turning a Spider Web Into a Musical Instrument

Video

Scientists report that they have translated the structure of a web into music, which could have applications ranging from better 3D printers to cross-species communication and otherworldly musical compositions.

WATCH NOW

New Theory Suggests the Brain Is a Giant Supercomputer

Video

Dr Ben Goult, a researcher from the University of Kent's School of Biosciences, has proposed a new theory for understanding brain and memory function. It suggests that the brain is a giant supercomputer, running a form of binary code. Neuroscience News and Research spoke to Ben to find out more.

WATCH NOW

 

Like what you just watched? You can find similar content on the communities below.

Genomics Research Informatics

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE