Here we adapt digital droplet PCR to operate under COLD-PCR conditions, thereby preferentially amplifying mutation-containing sequences in droplets. COLD-PCR enables amplicons with mutations at any position to be amplified preferentially, while wild-type samples are preferentially suppressed. The preferentially amplified sequences can subsequently be sequenced. We validate the merging of technologies in serially diluted TP53 and KRAS mutated sequences. By merging COLD-PCR and digital -PCR technologies we enable digital mutation scanning, a new frontier for digital-PCR. A single reaction can now interrogate numerous mutations, and replaces multiple individual digital-PCR reactions.
Knowledge-Based Identification of Plant Stress Regulators
Video Jan 19, 2015
The genetic code of all 500,000 UK Biobank volunteer participants will be sequenced by researchers at the Wellcome Sanger Institute in the UK and deCODE genetics in Iceland. This project is the single most ambitious sequencing programme in the world undertaken by a public-private partnership, and will power the next wave of genetic and health researchWATCH NOW