Here we adapt digital droplet PCR to operate under COLD-PCR conditions, thereby preferentially amplifying mutation-containing sequences in droplets. COLD-PCR enables amplicons with mutations at any position to be amplified preferentially, while wild-type samples are preferentially suppressed. The preferentially amplified sequences can subsequently be sequenced. We validate the merging of technologies in serially diluted TP53 and KRAS mutated sequences. By merging COLD-PCR and digital -PCR technologies we enable digital mutation scanning, a new frontier for digital-PCR. A single reaction can now interrogate numerous mutations, and replaces multiple individual digital-PCR reactions.
Knowledge-Based Identification of Plant Stress Regulators
Video Jan 19, 2015
Mycotoxins and Climate Change - How Europe Contributes to Global EffortsVideo
Mycotoxins are poisonous chemicals produced by moulds. They can contaminate food and feed crops such as cereals and nuts. Climatic conditions affect mycotoxin production so climate change is impacting the way we manage mycotoxin risks for human and animal health.WATCH NOW