Here we adapt digital droplet PCR to operate under COLD-PCR conditions, thereby preferentially amplifying mutation-containing sequences in droplets. COLD-PCR enables amplicons with mutations at any position to be amplified preferentially, while wild-type samples are preferentially suppressed. The preferentially amplified sequences can subsequently be sequenced. We validate the merging of technologies in serially diluted TP53 and KRAS mutated sequences. By merging COLD-PCR and digital -PCR technologies we enable digital mutation scanning, a new frontier for digital-PCR. A single reaction can now interrogate numerous mutations, and replaces multiple individual digital-PCR reactions.
Knowledge-Based Identification of Plant Stress Regulators
Video Jan 19, 2015
Garbage In = Garbage Out: Limited Peer Review in Cannabis SequencingVideo
Many companies are offering DNA sequencing for the cannabis field but not all of these companies have scientific staff that have published genomics papers. Do they know how to sequence? This recent review implies, in some cases, they do not.WATCH NOW