Here we adapt digital droplet PCR to operate under COLD-PCR conditions, thereby preferentially amplifying mutation-containing sequences in droplets. COLD-PCR enables amplicons with mutations at any position to be amplified preferentially, while wild-type samples are preferentially suppressed. The preferentially amplified sequences can subsequently be sequenced. We validate the merging of technologies in serially diluted TP53 and KRAS mutated sequences. By merging COLD-PCR and digital -PCR technologies we enable digital mutation scanning, a new frontier for digital-PCR. A single reaction can now interrogate numerous mutations, and replaces multiple individual digital-PCR reactions.
Knowledge-Based Identification of Plant Stress Regulators
Video Jan 19, 2015
Professor Sir Doug Turnbull from the Wellcome Trust Centre for Mitochondrial Research at the University of Newcastle explains his research into mitochondrial donation, the innovative treatment that hopes to stop faulty mitochondria being passed on from mother to child to prevent incurable genetic diseases.
The first babies conceived with this treatment through IVF may be born in the UK soon.
Scientists at the University of Sussex are working on the prototype of a quantum computer that could change the finance industry as well as medicine and cybersecurity.
This tour through their lab explores how the trapped ion quantum computer works and how it could revolutionise our lives.
From their diet to their diseases, koalas are pretty special. Now researchers have sequenced the koala’s genome, unlocking the secrets that make these fuzzy fellas so unique. The genome is revealing everything from how koalas cope with munching poisonous eucalyptus leaves, to how they respond to chlamydia infections. The hope is that these insights will not only help us understand these fascinating marsupials, but also aid conservation efforts across Australia.