Modular Human Biochip-based Organoid Models in Biomedical Research

Video   Oct 18, 2016

 

Animal models are frequently used in biomedical research. However, recently a controversial debate about the transferability of data obtained in mouse models to human conditions emerged. Although cell-based in vitro approaches can be an alternative, conventional cell culture methods hardly reflect cellular cross-communication and neglect essential physiological parameters of the living organism. We therefore develop biochip-embedded organoid models of the liver, gut and brain for use in basic medical research. The organoids are composed of essential human cell types of the respective organs and were tested in the context of sepsis-related organ dysfunction. Dynamic perfusion of biochip-embedded tissues allows an optimal supply with nutrients and oxygen, an efficient removal of catabolic metabolites, and enables a physiological cell polarization and communication within tissues. The modular approach of the biochip design allows a variable microfluidically interconnection of different organoid models in a freely selectable arrangement. Most important, the cellular processes observed under pathophysiological conditions in vitro closely resemble pathophysiological responses of established mouse models as well as clinical observations in human sepsis. Thus biochip-based organoid models are suitable experimental tools to improve study options on pathophysiological mechanisms of sepsis-related multi-organ failure and the development of targeted treatment strategies.

 
 
 
 

Recommended Videos

Blood, Rats and Anticoagulants: The Story of Warfarin

Video

The history of warfarin, one of the earliest anticoagulant drugs, is littered with the bodies of sick cows and poisoned rats. This animation tells the story of how a bloody beginning gave rise to a life-saving medication.

WATCH NOW

Introduction to Peptides and Proteins for Bioanalysis Using LC-MS

Video

Khalid Khan, Senior Manager Business Development, discusses the basic structure of amino acids, peptides, and proteins, including specific examples like monoclonal antibodies. The ionization and fragmentation of peptides and how they differ from small molecules is also discussed.

WATCH NOW

Solutions for High-Throughput and Phenotypic Screening

Video

Accelerate drug discovery workflows with state-of-the art imaging and detection instruments, assay technologies, reagents, versatile automation systems, and powerful informatics solutions that work together to ensure consistent, accurate, physiologically relevant results. Wrest more meaning from your findings with PerkinElmer's one-of-a-kind analytics, and amplify your phenotypic or target-based screens to identify hits and lead compounds in small and focused or high-throughput volumes.

WATCH NOW

 

Comments | 0 ADD COMMENT

Like what you just watched? You can find similar content on the communities below.

Cell Science Drug Discovery

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE