We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

Novel Chip-based Extraction of miRNA

Video   Jun 10, 2015

 



MiRNAs are small (20 to 23 nucleotides in length) non-coding RNAs that regulate numerous essential cell functions. They operate by targeting messenger RNAs for cleavage or translational repression and it is known that their expression profiles classify human cancers. In this study we present the development of a fast on-chip miRNA extraction system. Due to the size-limiting effect of this process, ribosomal RNA (rRNA), messenger RNA (mRNA) or genomic DNA are excluded. Both the duration and efficiency of the on-chip extraction process are compared to gold standard commercial extraction kits. On-chip lysis of e.g. breast cancer cells is achieved within only 30 - 45 seconds. MiRNAs are extracted from the crude lysate within 8 minutes (duration of commercial kit extraction: ~1h). Extracts are checked for purity by the Bioanalyzer 2100 capillary electrophoresis system. The excellent amplifiability of the extracted miRNAs is demonstrated by a RT-qPCR assay lacking preamplification steps. All on-chip processes are performed without the use of any toxic components.

 
 
 
 

Recommended Videos

Introduction to nanopore sequencing

Video

Oxford Nanopore Technologies has developed nanopore-based DNA and RNA sequencing technology designed to provide on-demand biological information, to any person, in any environment.

WATCH NOW

Cas13: Cas9's Cool Detective Cousin

Video

This animation depicts how Cas13—a CRISPR-associated protein—may be adapted to detect human disease. This new diagnostic tool, called SHERLOCK, targets RNA (rather than DNA), and has the potential to transform research and global public health.

WATCH NOW

How Do Pregnancy Tests Work?

Video

Over-the-counter pregnancy tests give potentially life-changing results with a pretty high rate of accuracy. But how do they work? Watch this video to find out.

WATCH NOW

 

Like what you just watched? You can find similar content on the communities below.

Diagnostics Genomics Research

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE