One of the challenges in determining the collective function of these tissues and organs is in defining the functions and contributions of the different cell types. We have extensively developed and deployed methods for analysis of gene expression within different cell types based on the use of flow cytometry and cell sorting, coupled to the regulated expression and targeting of Fluorescent Protein markers within specific cell types. This presentation will discuss these methods, and their results, and will go on to consider the extent to which they can be applied to the analysis of single cells. The ability to measure transcription within individual cells raises and identifies the issue of stochasticity within the overall framework of a shared pattern of gene expression within specific cell types and, together with the recent emergence of evidence for genomic variability within somatic cells, predicts the existence of further regulatory levels that constrain cell function.
Novel Technologies for Studying Functional Genomics at the Level of Single Cells
Video Jan 05, 2015
Meet the biohacker who wants to teach everyone how to edit genes.
Josiah Zayner is a biohacker who thinks everyone should be able to change their DNA with biotechnology called CRISPR. That’s why he founded a company called The ODIN, which sells do-it-yourself biotech kits that teach people how to genetically modify bacteria and frogs. It's DIY gene therapy.
His company has sold tens of thousands of experiments using CRISPR, an inexpensive and precise gene-editing technology that has revolutionized the field.
Science really is everywhere! From the Big Bang to quantum mechanics to free will to ice skating, we explore it all featuring Neil deGrasse Tyson, Chuck Nice, theoretical physicist Brian Greene, neuroscientist Heather Berlin, rapper Baba Brinkman, and Olympic silver medalist figure skater Sasha Cohen.WATCH NOW