Overcome FFPE Struggles in WGS: A Proven Path to High-Confidence Data
Formalin-fixed, paraffin-embedded (FFPE) samples pose persistent challenges for whole-genome sequencing (WGS) due to fragmentation, crosslinking and chemical modifications. Labs often struggle with low DNA yield, inconsistent insert sizes and unreliable variant calling.
In this Science Spotlight episode, Bella Pfeiffer, an Automation Research Associate at Covaris, explains how Adaptive Focused Acoustics® (AFA®) workflows overcome these obstacles, enabling higher recovery and more confident sequencing results.
Watch this episode to explore:
- How AFA® improves DNA extraction and library prep from FFPE tissue
- Strategies for retaining usable sequencing reads and uniform insert sizes
- Practical workflow tips for high-confidence whole-genome sequencing
Further resources
- truCOVER® Library Preparation Kits
- High-Fidelity Whole-Genome Sequencing From Low-Input, Formalin-Fixed Paraffin
- Optimization of DNA Fragmentation Techniques To Maximize Coverage Uniformity of Clinically Relevant Genes Using Whole Genome Sequencing
- Covaris truCOVER® Library Prep Kit: A Streamlined, PCR-Free WGS Workflow for Enhanced NGS Library Performance and Data Quality
- Unlocking Precision in WGS Through Better Library Preparation