We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

Plasma Vitronectin Predicts Response to Fluoropyrimidine-oxaliplatin (FOLFOX) Chemotherapy in Metastatic Colorectal Cancer

Video   Jul 21, 2015

 

Current assessment is based on computed tomography scans, approximately 6-8 weeks after treatment. Earlier molecular predictors of response would be highly beneficial for both patients and clinicians. We have investigated whether useful protein biomarkers could be found in patient plasma, through the use of an advanced MS technique known as SRM-MS. Here, we will discuss the discovery-through-validation pipeline of identifying and validating clinically-relevant biomarkers that can be rapidly translated into the clinic. Briefly, we examined whether the type of blood collection tube would impact on SRM measurement of plasma proteins, in addition to examining the stability of the selected plasma proteome over a 19 week time-course. We then applied SRM to monitor 31 plasma proteins in a trial cohort of advanced CRC patients receiving FOLFOX chemotherapy. Our results indicated that the baseline levels of plasma vitronectin were associated with chemotherapy response, which was successfully validated in a larger patient cohort using SRM and ELISA (n=29 patients). These findings pave the way for further testing in larger cohorts and could lead to more cost-effective and rational uses of systemic chemotherapy.

 
 
 
 

Recommended Videos

How to Capture Images with Invitrogen iBright Imaging Systems

Video

Capture images and analyze data from your western blots and gels efficiently and easily using the Invitrogen iBright Imaging Systems.

WATCH NOW

Cell Culture Basics

Video

An overview of aseptic techniques

WATCH NOW

Should You Eat a Low-gluten Diet?

Video

When healthy people eat a low-gluten and fibre-rich diet compared with a high-gluten diet they experience less intestinal discomfort including less bloating, a new study shows. The researchers attribute the impact of diet on healthy adults more to change in composition of dietary fibres than gluten itself.

WATCH NOW

 

Like what you just watched? You can find similar content on the communities below.

Analysis & Separations Cancer Research Diagnostics Proteomics & Metabolomics

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE