The Real Sources of Bias in Low-Input WGS – Rethinking Fragmentation, GC Content and Input Variability

Whole-genome sequencing is essential for genomics research, but preparing high-quality libraries from low DNA inputs is a growing challenge in applications such as oncology and liquid biopsy. Ultra-low DNA inputs often lead to uneven coverage and reduced variant accuracy.

Covaris’ truCOVER® workflow uses Adaptive Focused Acoustics® (AFA®) to simplify library preparation while ensuring consistent results across sample inputs – even those in the sub-nanogram range. 

In this Science Spotlight episode, Vanessa Process, a scientist at Covaris, shows how truCOVER® supports high-quality WGS for advanced research and clinical applications.

Watch this episode to understand: 

• How AFA® shearing maintains coverage uniformity and minimizes GC bias
• How low-input amplification supports accurate variant calling
• Applications in single-cell genomics and metagenomics

Further Resources:

• To learn more about truCOVER® Library Prep, visit https://www2.covaris.com/truCOVER/AMP Or contact Covaris at [email protected]
•  How to use the truCOVER® WGS Library Prep workflow paired with an optional DNA Amplification Module 
• Demonstration of the the truCOVER® WGS workflow with the Amplification Module
• How mechanical fragmentation with Covaris AFA® and truCOVER® Library Prep delivers lower GC-bias and more uniform coverage than enzymatic kits 
• Covaris truCOVER™ DNA Library Prep Kits for unmatched flexibility and performance
• Greg Endress explains how mechanical shearing and streamlined single-vessel workflows are enhancing the accuracy, uniformity and scalability of WGS.