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Unlocking Deeper Insights Into RNA Biology With Full-Length Transcripts

Full-length RNA sequencing goes beyond gene-level counts—it reveals the isoform structure and modifications hidden within transcripts, helping researchers uncover biology that short-read approaches often miss.

In this episode of Teach Me in 10, Patrick Murphy, Market Segment Manager for Transcriptomics at Oxford Nanopore Technologies, explains how full-length reads give researchers a more complete view of the transcriptome. He walks through why short-read sequencing struggles to resolve isoforms across multiple exons, how full-length reads capture entire transcript structures in a single read, and how direct RNA sequencing can reveal modifications that short-read methods can't detect.


Watch this episode to explore:

  • What full-length reads can offer for transcriptomics that short reads cannot 
  • The benefits of direct RNA sequencing for detecting modifications 
  • How Oxford Nanopore RNA sequencing performance compares to alternative approaches


Further resources: