Case Study: Improved Quantitative Sensitivity with Novel Microflow LC and MS Source System

Webinar

 
Case Study: Improved Quantitative Sensitivity with Novel Microflow LC and MS Source System
 
Chris Holliman
Sr. Director, Global, Small-Molecule, Bioanalytical Pfizer, Groton CT
Joseph Tweed
Senior Principal Scientist Pfizer, Groton CT
Jason Barricklow
Scientist Pfizer, Groton CT
 

LC-MS/MS
is the standard technique for the highly selective and sensitive
quantitation of small and large molecule therapeutics in biological
matrices. However, current strategies used in the development of
pharmaceuticals present a challenge for even the most sensitive LC-MS/MS
platforms.


One route to improve assay quantitation limits is to reduce the eluent
flow to the electrospray ionization source with microflow
chromatography.


This webinar will focus on the evaluation and use of the new SCIEX
OptiFlow Quant Solution, with M5 MicroLC system and novel OptiFlow MS
source to improve quantitative assay sensitivity.


Attend this webinar to discover


  • The advantages of reducing eluent flow to the electrospray
    ionization source, and the challenges experienced in transitioning to
    microflow LC-MS/MS assays
  • How different approaches in microflow chromatography can be employed in both discovery and regulated laboratories
  • how sensitivity gains of 3-10X can be achieved when the LC
    analytical flow is reduced from conventional flow rates to the
    micro/capillary flow rates of 2-10 uL/min.

 
 
 
 

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