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Applications of Droplet Digital PCR


Applications of Droplet Digital PCR

The use of DNA as a biomarker relies on analysis tools that are highly sensitive and give reproducible results.  Digital PCR is a newer technology for DNA detection and quantification that is rapidly being adopted for applications in precision medicine.  This on demand seminar consists of 4 talks covering which will cover the following topics:

  • Digital PCR basics and assay development
  • The application of digital PCR to disease diagnostics
  • Digital PCR for highly sensitive non-invasive prenatal testing (NIPT)
  • Cell-free DNA cancer testing with digital PCR

Talk 1: Molecular Diagnostics: ddPCR for rapid RHD zygosity determination and highly sensitive non-invasive prenatal testing (NIPT) of fetal sex and RHD genotype presented by Dr Kelly Sillence, Plymouth University

Talk 2:  Development of Droplet Digital PCR based Diagnostic Tests for mutations in three bone tumours: Chondrosarcoma (IDH1), Giant Cell Tumour of the bine (H3F3A) and, Fibrous Dysplasia (GNAS1) presented by Manu Gupta, Sarcoma Genetics Group, Research Department of Pathology, UCL

Talk 3: Comparison between Droplet Digital PCR and real time PCR: Methylation at the Limit of Detection presented by Benjamin Brown, University of Liverpool Cancer Research Centre, Roy Castle Lung Cancer Research Programme

Talk 4: Standardisation of innovative cfDNA assays, an essential ingredient: controls! Presented by Kasia Wilczynska, PhD, Senior Scientist, Horizon Discovery


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