Epitranscriptome screening for the characterization of the pseudouridine landscape on mammalian RNA
Dr Schraga Schwartz
Senior Lecturer, Weizmann Institute of Science
Product Manager, Twist Bioscience
Following synthesis, RNA can be modified with over 100 chemically distinct modifications, which can potentially regulate RNA expression post-transcriptionally. Pseudouridine (Ψ) was recently established to be widespread and dynamically regulated on yeast mRNA, but less is known about Ψ presence, regulation and biogenesis in mammalian mRNA. Dr. Schraga Swartz will discuss his work, which sought to characterize the Ψ landscape on mammalian mRNA, to identify the main Ψ-synthases (PUSs) catalyzing Ψ formation, and to understand the factors governing their specificity towards selected targets.
Key Learning Objectives:
- Learn a novel screening approach to characterization of RNA modification
- Understand the pseudouridine landscape on mammalian RNA