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High-Throughput Quantitative Analysis of Bispecific Antibody Moieties and Glycosylation


High-Throughput Quantitative Analysis of Bispecific Antibody Moieties and Glycosylation
Wilson Phung
Scientific Researcher, Genentech
Eric Carlson, Ph.D.
President and CEO, Protein Metrics

Bispecific antibodies are of great interest as potential therapeutics because they can bind to two different targets simultaneously.  Their formats, based on IgG, are preferred for many applications, however there are many hurdles to bispecific production.

In this webinar, Wilson Phung will explain how to solve technical hurdles to bispecific production using an LC-EMR workflow that provides high-throughput sample processing for BsAb quantification.   He will demonstrate how to overcome analytical challenges to resolve impurities, deconvolution, and quantification of half mAb and full mAb by UV, ultimately leading to the final selection of the bispecific antibody variant.   He will also discuss how to characterize antibody glycosylation with a site-specific glycosylation analysis workflow.

Key Learning Objectives:

  • Achieve higher throughput workflows to alleviate the mass spec data analysis bottleneck
  • Eliminate unwanted heavy chain homodimerization, and unwanted light chain, heavy sample miss-pairs
  • Select optimal clones for stable cell lines
  • Analyze site-specific glycosylation and other post-translational modifications
  • Overcome complex analytical and reporting challenges


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