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Colorful fluorescence peaks illustrate multiplexing in real-time PCR.

Multiplexing in Real-Time PCR

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Developing a multiplex real-time PCR assay requires deciding on the number of targets, checking assay compatibility, choosing reporter dyes, and testing performance. Making the right decisions early can help researchers determine whether multiplexing is appropriate and establish an effective strategy for development and testing.


Attendees will gain a clearer understanding of the factors that influence multiplex design and learn how to approach development with greater confidence.

Attend this webinar to: 

  • Learn when multiplexing is favorable for real-time PCR applications
  • Understand how to use the TaqMan™ multiplex interaction check tool that helps assess assay sequence interactions before ordering or testing
  • Discover how reporter dye selection and primer concentrations can support effective multiplex assay design
  • Gain insight into how performance testing can help ensure a highly successful multiplex
Speaker
A picture of John B. Pfeifer, PhD
John B. Pfeifer, PhD
Sr. Staff Scientist, Field Applications
Thermo Fisher Scientific
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