Polishing Strategies for Full Adeno-Associated Capsid Enrichment

Polishing Strategies for Full Adeno-Associated Capsid Enrichment This webinar provides a framework for optimizing AEX polishing strategies to improve AAV full capsid enrichment. Through AAV2, AAV9, and a high-throughput screening case studies, speaker Chiu Lau-Barre will discuss how the selection of buffer systems, salt chemistry, conductivity, and elution design can address different serotype and capsid-specific behavior and influence enrichment, recovery, and scalability. As adeno-associated virus (AAV) therapies advance toward larger-scale manufacturing, achieving consistent full capsid enrichment remains a critical challenge. Variability between serotypes and purification conditions can affect product quality, recovery, and process scalability. In this webinar, Chiu Lau-Barre will share practical strategies for developing robust anion exchange (AEX) polishing workflows, drawing on real-world AAV2 and AAV9 examples. Attendees will gain insight into approaches for improving purification performance, streamlining process development, and supporting efficient scale-up. The session will also highlight how modern screening tools can help accelerate decision-making and reduce development timelines. Attend this webinar to: Understand key variables that influence AEX polishing performance of full/empty capsid separation for AAVs Explore AAV2 and AAV9 case studies demonstrating how AEX polishing methods can be optimized across serotypes Learn how high-throughput AEX screening can accelerate process development and support scalable workflow implementation Identify process development strategies that can help reduce optimization timelines and support scale-up readiness Chiu Lau-Barre Staff Scientist, Field Applications Thermo Fisher Scientific