Revealing the secrets of nanodisc architecture using multi-detection GPC/SEC
Membrane protein research is dependent on the availability of membrane mimics that retain the structure and function of the target protein within a lipid-bilayer environment while rendering it amenable to in vitro investigations.
Styrene/maleic acid (SMA) copolymers extract phospholipids and proteins from artificial and natural membranes to form polymer-bounded nanodiscs (SMALPs) which retain the lipid-bilayer architecture of the host membrane. Therefore, SMALPs are gaining increasing interest among membrane-protein researchers as an attractive alternative to conventional membrane mimics such as micelles and liposomes. However, the morphology of SMALPs, and, in particular, the extent to which they provide a “protein-friendly” lipid-bilayer environment, remains poorly characterized.
In this webinar, we will demonstrate how multi-detector GPC/SEC provides compositional and morphological information on SMALPs, contributing to a better, quantitative understanding of these new membrane-mimetic nanoparticles.