RT2 Profiler™ PCR Arrays: Pathway-Focused Gene Expression Profiling with qRT-PCR

This paper evaluates the performance of the newest technique for monitoring the expression of a panel of pathway- or disease-specific genes: the RT² Profiler PCR Array System from SA Biosciences. The PCR Array System combines the quantitative performance of SYBR® Green-based real-time PCR with the multiple gene profiling capabilities of a microarray. The PCR Array is a 96- or 384-well plate containing RT² qPCR Primer Assays for a set of 84 related genes, plus five housekeeping genes, and three controls. A complete system includes an instrumentspecific master mix and an optimized first strand synthesis kit. This paper presents scientific data showing that PCR Arrays have the sensitivity, reproducibility, and specificity expected from real-time PCR techniques. As a result, this technology brings focused gene expression profiling to any biological laboratory setting with a real-time PCR instrument.