Protein loss during sample preparation can be an obstacle to reliable product quantitation in all biopharmaceutical settings. Samples are often prepared from feedstocks containing insoluble particles and therefore require a solid-liquid separation before analysis to protect analytical instruments. Because separation by centrifugation requires a difference in density between solid and liquid phase, sample filtration can be advantageous and membrane filters offer absolute particle retention. However, filter membranes can adsorb analytes like proteins and thereby distort the results of the subsequent analyses.
Download this app note to learn how to select the best filter membrane to:
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